Oxyma-based phosphates for racemization-free peptide segment couplings
نویسندگان
چکیده
منابع مشابه
Oxyma-B, an excellent racemization suppressor for peptide synthesis.
Peptide-bond formation is a key process in the synthesis of peptide oligomers. Among the many coupling techniques reported, carbodiimides combine strong acylation potency and smooth reaction conditions and are commonly used in the presence of additives. Recently, ethyl 2-cyano-2-(hydroxyimino)acetate (OxymaPure) has emerged as a highly reactive alternative to the classic and explosion-prone ben...
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We report the establishment of the O-acyl isopeptide method-based racemization-free segment condensation reaction toward future chemical protein synthesis. Peptide segments containing C-terminal O-acyl Ser/Thr residues were successfully synthesized by use of a lower nucleophilic base cocktail for Fmoc removal, and then coupled to an amino group of a peptide-resin without side reactions or epime...
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Oxyma [ethyl 2-cyano-2-(hydroxyimino)acetate] has been tested as an additive for use in the carbodiimide approach for formation of peptide bonds. Its performance in relation to those of HOBt and HOAt, which have recently been reported to exhibit explosive properties, is reported. Oxyma displayed a remarkable capacity to inhibit racemization, together with impressive coupling efficiency in both ...
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The aim of this review is to cover recent advances in acylation chemistry (peptide, amide, and ester bond formation) in which ethyl 2-cyano-2-(hydroxyimino)acetate (Oxyma)-derived reagents are involved. The distinct applications of each class of Oxyma derivatives will be discussed and compared to the behavior of reputed standards in the field. Outline
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Molecular farming technology offers a unique advantage that almost any protein can be produced economically and safely under very controlled conditions. Besides traditional production systems, such as bacteria, yeasts, insects and mammal cell lines, plants can now be used to produce eukaryotic recombinant proteins, especially therapeutic ones. Their advantages as hosts for protein production in...
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ژورنال
عنوان ژورنال: Journal of Peptide Science
سال: 2016
ISSN: 1075-2617
DOI: 10.1002/psc.2859